Life Advice Looking Through A Window

Power of chair

We all want to see ourselves strong. Often there comes a time when we think that I wish we could do this, I wish we had this power. One such power is the strength of the chair but it is the most special. Getting it can change the fate of generations, but somewhere we are all afraid of it, all the battles of the world have been done for it. All the freedom struggles have happened just to bring the power of the chair to the people.

Often the power of the chair is considered to be the power of the politicians, but it’s the strength of every person who has any position. A H.R. have the power of the H.R.s chair. Simply we can put it as, every person who has any position or responsibility has that chair of power.

There is a famous quote “With great power comes great responsibility”. Once everyone start treating it as responsibility rather than as a power world will have no fights.

Application of DNA Recombinant Technology in cloning

a. Background:
From the inception and evolution of mankind we have been able to make groundbreaking progress in the field of medicine but still some inborn genetic diseases like cystic fibrosis, hemophilia and familial hypercholesterolemia had no proper cure till a few decades ago. The field of Recombinant DNA technology opened a new world of possibilities.


b. Rationale of the study; Why is this study important?
Genes are a section of DNA which determine almost everything about our body. They determine things from our appearance , survival , health and behavior . Any malfunctioning or bad genes inherited or mutated can cause life hampering illness Using Recombinant DNA technology most recent and powerful tool CRISPR which has revolutionized and making gene editing possible and most importantly affordable and carried out in a simpler way treatment of these diseases by altering the disease causing gene sequence has been made possible.

c. Results & Conclusion of the research
Gene therapy is the referred to the process of introducing genes into existing target cells to prevent or cure a variety of diseases .For achieving the goal of delivering these genes effectively is accomplished by 2 methods:

i. Gene Delivery Vectors: For purpose of gene cloning at scale nothing is better than
viruses since they have machinery to replicate exponentially and deliver to cells .
Retroviral vectors are made by converting RNA genome to DNA in infected cell so they
are able to integrate host genome . By manipulating the viral genome viral


ii. CRISPR: In 1987 while investigating a particular gene in e coli scientists noticed a pattern in DNA surrounding it 5 identical sequences each composed of 29 bases separated from each other by 32 base block of DNA called spacers with unique sequence These repeating patterns were found in many different microbes and was coined the term Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR).


iii. They were found next to enzyme producing genes known as CRISPR Associated Genes (Cas Genes) which can cut DNA .
iv. Later found that these were a technique used by Bacteria to protect against viral invasions by saving part
of viral DNA in CRISPR sequence and spacers contains history of all infection. Upon attack of
virus again CRISPR sequence is transcribed in crRNA molecules which binds with
matching viral DNA which is then cut out by CAS9 enzyme.


v. Later it figured that CRISPR /CAS9 system is programable and can be used for gene
editing by synthesizing guide RNA molecule which match the sequence of the gene
which needs to be edited then the CAS9 enzyme cuts the matching DNA sequence on
both ends .


vi. In 2015 it was used to cut HIV virus out of living cells in lab and later on rats infected
with HIV were successfully treated .


vii. In 2019 a patient named Victoria Gray suffering from sickle cell disease caused due to
sickle shaped deformed hemoglobin was the first patient cured using CRISPR editing

Using CRISPR is a new breakthrough achievement which can provide a better life to
many others like Victoria Gray who suffer from these life threatening diseases as in US alone there
are 100,000 people who suffer from this disease as it can be inherited.

How satellite data can make companies rich

“Data is the new Fuel” Every company needs data, but now they are no longer dependant on digital resources, now they can get actual real world data, you can never ask your costumer what are they going to purchase after purchasing your product or why they prefer your competitors product over your’s, but now you can know and multiply your profit heavily. 

For a long time satellite data was on military’s hold but now things are changing, with advances of AI and ML now everyone can utilize satellite imagery for themselves and in there own unique way and governments are supporting the same. 

Here’s some facts from allied market research

Satellite Data Services Market to Reach $19.36 Bn, Globally, by 2027 at 19.1% CAGR: Allied Market Research”

Now how you can utilize

Data- the biggest profit is data, the more data you have the more profit you get. Companies are using techniques like web scraping to get data, know their market, and competitor; and you can use it as same but it’s real world data, how much customers are coming in your or your competitors firm or even how much they are earning. 

Surveillance-  you can have an eye on product delivery and management so you always know on-ground data, except for softwares products it works everywhere. 

Security- you can ensure the safety of products way ahead of CCTV era.

Here are some well known Indian firms to have you start

Dimension IGIS

Geokno

Geoinfosys

Names doesn’t stop here as it’s a growing industry, they will analyze and refine the data as per your need. 

Here’s link of some free isro courses to give more grasp. 

https://elearning.iirs.gov.in/edusatregistration/student

Always remember that in business, or anywhere one with the most data wins. In these era of competition you must always be equipped with latest tech to give your customer best, and satellite data is just what you need to have an edge. 

Research


From allied market research

https://www.prnewswire.com/news-releases/satellite-data-services-market-to-reach-19-36-bn-globally-by-2027-at-19-1-cagr-allied-market-research-301206045.html

Download Report Sample (252 Pages PDF with Insights, Charts, Tables, Figures) at https://www.alliedmarketresearch.com/request-sample/6793

Serverless does it have servers?

Serverless does it have servers?

Or it has something more exciting.

For those who know about cloud or are interested about it must have heard this word once “serverless”. As confusing as it sounds it has created a lots of myths around this like

Is serverless actually server less?

Is serverless and FaaS really same?

If you are curious about this or just wanna know serverless let’s find out together. Also we will be taking some important questions like why you should learn/know serverless, does your enterprise actually need serverless? Let’s find out together.

The first thing is what is serverless-

According to cloudflare- serverless computing is a method of providing back-end services on an as-used basis.¹

Also serverless framework is free opensource framework build for developing lambda a serverless computing platform of amazon. So what serverless basically means is rather than managing your server together with application you let the server provider manage it. This way you can focus more on application you are writing. You give them the code and they will imply this on server and will charge you based on traffic and services. so serverless doesn’t means having no server but means having no hussle of servers.

Many times FaaS and serverless are taken as replaceable words but are they same? 

While serverless is a broad term Serverless computing can be divided in two types

BaaS(back-end as a service)

FaaS(function as a service)

so FaaS is a type of serverless computing.

Now why should you learn/know serverless? It’s simple as it’s a growing field if you want to use it in firm, in your career or if you just want to feed your curiosity you should learn/know it.

Does you enterprise or you need it?

The big part of you decision depends on

Time- if you have time to manage back-end issue or you love doing it you can leave serverless

Money- It also depends on how well you can manage finances.

Knowledge- Also you should know you or the  human resource you hold how well they have knowledge about back-end or functions or will it be better leaving on serverless.

Hope this feeds your queries.

10 best place to learn serverless

1 . Serverless cloud development by qwicklabs

Qwicklabs is always my #1 resource for learning something cloud, for these article too. Its bitesize lessons makes it easy to grasp or remember concept even if you are taking quest or not.

Remember you must sign in to read it’s content

https://www.qwiklabs.com/quests/152

2 . Serverless full stack application on aws- serverless.com

It’s a tutorial or a course by serverless.com to teach about serverless and its implications for full stack development and various tricks to utilise it financially 

https://www.serverless.com/learn/courses/full-stack-application-development-on-aws/

3. Microsoft learn

It has bite-size lessons, quizzes, some exercises, cute badges, you can always look how much you learnt or can take a quiz to refurbish your knowledge but most importantly a community to help with they want to try the stuff as the content progress for better grasping.

https://docs.microsoft.com/en-us/learn/paths/create-serverless-applications/

4 . 7- best serverless courses and aws lambda courses to learn in 2021-      medium

It’s a article by medium in which it explains what is serverless and why it’s important but also connects to lots of important resources to further proceed.

https://medium.com/javarevisited/7-best-serverless-and-aws-lambda-courses-to-learn-in-2021-de1820111c85

5 . Serverless- free code camp.org

 A collection of 73 posts on code camp. You can read them to understand serverless and what’s going on latest in it. 

https://www.freecodecamp.org/news/tag/serverless/

6. Serverless computing solutions Google cloud

It’s Google cloud explaining what’s serverless and why you should use there serverless products. This article is nice for those who are new and want to get start, it has a video, some links to documentation, some other sources like there trusted partners and some examples.

https://cloud.google.com/serverless

7. What is serverless computing?- IBM cloud education

It’s IBM C.E. explaining serverless, it’s nice but as it’s from IBM C.E. it’s more of clearing concepts and confusions and getting little learner insight instead of promotion of service like in above article, it’s worth reading for students looking for serverless or wants to clear there basics.

https://www.ibm.com/cloud/learn/serverless

8. What is serverless- gitlab

It’s a nice article by gitlab giving Idea of what is serverless and several open-source and big firm products you can get.

https://about.gitlab.com/topics/serverless/

9. Serverless- the new stack

It’s a short 1 minute article discussing what big brains think about serverless, what it is, it’s profits and challenges.

https://thenewstack.io/category/serverless/

10. What is serverless- cloudflare

It’s language is comparatively simple to above articles and it provides detail insights of serverless, if you’re jumbling in tough tech words can give this article a try.

https://www.cloudflare.com/en-in/learning/serverless/what-is-serverless/

5 reasons why working in startup is good idea



If you are confused whom to choose MNC or startup here’s my 5 reason why it should be a startup.

Learning
While you can learn and get help from your nice colleagues in MNC, at startup you can learn a lot other interesting things.

Pushing the limits
As a startup they need everyone to push there limits to find there new strength as this may not be available on established MNCs, you might wanna start at a startup.

Community
You might get some friends in MNC but while it comes to startup most of them wants to start, needs help and are willing to help, making it a nice community.

No boss
Startup too have bosses but as they are in early phase  they are more of a friend, helper and mentor, so if you are new in office work you might wanna choose startup first.

Future
If your startup grows you too will get credit of it unlike in MNCs you can use it in CV and show how much it has grown and what are the role you played in its growth.

This are not the only reasons sometimes they can give you lifelong friends, experience and if you are dedicated to work, journey in startup is lot more fun.

STRESS- CAUSES, EFFECTS,SYMPTOMS AND TREATMENT

BY DAKSHITA NAITHANI

INTRODUCTION

Stress is a human emotion that is natural and affects everyone at some point in their lives. The human body is built to recognise and respond to stress. An individual’s body creates physical and mental reactions in response to changes or difficulties (stressors). It is any sort of change that produces physical, emotional, or psychological pressure. It may be caused by a variety of conditions or life events. When we encounter anything new, unanticipated which challenges our sense of self, or when we believe we have little control over a situation, it is frequently activated.

Your body’s stress reactions assist it in adapting to new conditions. A stress reaction, for example, may help your body work harder and remain awake longer if you have an important test coming up. When stresses persist without release or times of relaxation, it becomes a problem.

To some extent, everyone is stressed. We all handle stress in various ways which also has a significant impact on your wellbeing .Genetics, early life events, personality, and social and economic conditions can all influence our capacity to cope.

TYPES OF STRESS

Not all types of stress are harmful or even negative. Some of the different types of stress that you might experience include:

Acute stress:  We face this type of stress in daily life it is short term and can be either positive or distressing.

 Chronic stress: Chronic stress is a type of stress that appears to be never-ending and unavoidable, such as the stress of a physically demanding profession; chronic stress can also result from catastrophic events and psychological trauma.

Episodic acute stress: Acute stress that appears to be rampant and becomes a way of life, resulting in a life of constant distress, is known as episodic acute stress.

Eustress: Eustress is a thrilling and enjoyable experience. It’s a type of positive stress that can help you stay energised. It’s linked to adrenaline rushes, as when you’re skiing or rushing to reach a target.

CAUSES OF STRESS

There are numerous factors that might generate stress in one’s life. Work, economics, partnerships, parenthood, and day-to-day hassles are all common causes of stress. Workplace stress may also be detrimental to your psychological health. Workplace stress causes people to miss an average of 24 days of work each year due to illness.

Even life changes that are positive in nature such as moving to a larger house, getting a work promotion, or going on vacation can lead to stress. If you’re anxious in certain situations, you could have a hard time understanding why, or you might be hesitant to express your thoughts with others.

The fight-or-flight response, which is usually triggered by stress, is the body’s reaction to a perceived threat or danger. The fight-or-flight response, which was called for its capacity to enable us to physically fight or flee when faced with danger when is triggered in situations where both the responses are not appropriate, such as in traffic or during a stressful day at work. The relaxation reaction does not occur frequently enough in instances of chronic stress, and being in a near-constant state of fight-or-flight might harm the body.

HOW CHRONIC STRESS AFFECTS YOUR HEALTH

When you consider the influence stress has on your life, you can see the link between your mind and body. Physical health problems might arise when you are worried out about a relationship, money, or your living circumstances. It’s also true in reverse. Wellness issues, whether you have high blood pressure or diabetes, will have an impact on your stress level and mental health.

Heart attacks, arrhythmias, and even sudden death can be triggered by severe acute stress, such as being involved in a natural disaster or getting into a verbal altercation. Stress has an emotional impact as well. While mild anxiety or frustration may result from some stress, prolonged stress can lead to burnout, anxiety disorders, and depression. When under a lot of stress, your autonomic nervous system becomes overactive, which can harm your body.

SIGNS AND SYMPTOMS

Continuous activation of the stress response creates wear and tear on the body. When a person is under stress for a long term symptoms manifest themselves in the form of physical, emotional, and behavioural manifestations.

Physical symptoms of stress include:

  • Aches and pains such as chest pain or a feeling like your heart is racing.
  • Feeling exhausted all the time or having trouble while sleeping.
  • Having Headaches, High blood pressure, dizziness or shaking.
  • Muscle tension or jaw clenching (Grinding teeth)
  • Having various digestive problems.
  • It also results in a weak immune system.
  • Clammy or sweaty palms

Stress can lead to emotional and mental symptoms such as:

  • Anxiety or irritability.
  • Depression.
  • Panic attacks.
  • Sadness.
  • Changes in mood

Identifying Stress

Having stress for long-terms has also been related to various gastrointestinal diseases including Irritable Bowel Syndrome (IBS) or stomach ulcers, as well as cardiovascular illness, according to certain studies.

Stress may originate from a variety of places, but even little everyday pressures from job, school, family, and friends can have a negative impact on your mind and body.

If you think it might be bothering you, there are a few things you can look for:

  • Difficulty concentrating, worrying, anxiety, and difficulties remembering are all psychological symptoms.
  • Anger, irritability, moodiness, and frustration are examples of emotional indicators.
  • High blood pressure, weight fluctuations, frequent colds or infections, and changes in the menstrual cycle and libido are among physical symptoms.
  • Poor self-care, not having time for the activities you like, or depending on drugs and alcohol to cope are all behavioural markers to look out for.

TREATMENT

There is no one particular therapy for stress because it is not a separate medical diagnosis. Change the environment, improve stress coping abilities, apply various relaxation techniques and treating conditions that may have been created by chronic stress are all part of stress treatment.

Psychotherapy, medication, and complementary and alternative medicine are some of the therapies that may be beneficial.

HOW TO COPE WITH STRESS

Stress is unavoidable, but can be managed. One can take control of their health and decrease the influence stress has on life once they understand the toll it takes and how to battle it. You can’t escape stress, but you can keep it from becoming overwhelming by using the following methods on a regular basis:

  1. Exercise

Regular exercise is one of the most effective methods to calm your body and mind. Plus it will also lift your spirits. Work up to 2 hours and 30 minutes of moderately intensive activity, such as brisk walks, or 75 minutes of rigorous exercise, such as swimming laps, running, or participating in other sports. Focus on creating realistic fitness goals so you don’t give up.

  • Relax Your Muscles

Your muscles stiffen up when you’re anxious. Stretching, getting a massage and other self-care activities can help loosen them up and rejuvenate your body. Taking a hot bath or shower is a great way to relax. Having a restful night’s sleep is also very important.

  • Deep Breathing

Stopping and taking a few deep breaths can immediately relieve stress. Once you get the hang of it, you’ll be amazed at how much better you feel. Simply take these five steps:

  • Sit with your hands in your lap and your feet on the floor in a comfortable posture.
  • Close your eyes for a moment.
  • Consider yourself in a soothing environment. It may be on the beach, in a lovely field of grass, anyplace else that helps you feel calm.
  • Slowly inhale and exhale deeply.
  • At a moment, do it for 5 to 10 minutes.
  • Eat Well

Eating a good well-balanced, regular diet will make you feel better overall. It may also aid with mood regulation. For energy, your meals should include plenty of vegetables, fruit, healthy grains, and lean protein and make sure you don’t forget any. 

  • Slow Down
  • 5 to 10 minutes ahead of time, set your watch. You’ll be able to arrive a bit earlier and avoid the worry of being late.
  • Switch to the slow lane if you’re travelling on the highway to prevent road rage.
  • Break down projects into manageable chunks. If you don’t have to, don’t try to respond to all 100 emails; instead, respond to a handful of them.
  • Take a Break

 To give your mind a break from stress, schedule some genuine leisure. It may be difficult for you at first if you are someone who enjoys setting objectives for the day. But if you stay with it, you’ll come to like these times. Meditation, Yoga, Tai chi, Prayer, Listening to music, and spending time in nature are all relaxing activities.

  • Make Time for Hobbies

You must schedule time for activities that you like. Every day, try to do something that makes you happy, it helps you to relax. It doesn’t have to take a long time; 15 to 20 minutes would suffice. Reading, knitting, doing an art project, playing golf, watching a movie, doing puzzles, and playing cards and board games are all relaxing activities.

  • Talk About Your Problems

If anything is troubling you, talking about it might help you relax. Family members, friends, a trustworthy priest, a doctor, or a therapist are all good places to start.

So, when you’re worried, pay attention to what you’re thinking or saying. Change the negative message you’re sending yourself to a positive one. Don’t tell yourself, “I can’t do this,” for example. Instead, tell yourself, “I can do this,” or “I’m doing the best I can.”

  • Go Easy On Yourself

Accept that no matter how hard you strive, you will never be able to achieve everything with perfection. So, do yourself a favour and quit being hard over yourself and stop overestimating your abilities. Also, remember to retain your sense of humour. Laughter is one of the most effective ways to relax.

  1. Eliminate Your Triggers

Determine the main sources of stress in your life. Is it your job, your commute, or your schooling that’s the problem? If you can figure out what they are you may get rid of them or at least decrease them in your life.

EXTREMOPHILES: SALINITY AND AT LOW NUTRIENT LEVELS

BY DAKSHITA NAITHANI

Prokaryotic life has dominated much of our planet’s evolutionary history, developing to fill nearly every possible environmental niche. Extremophiles are one of these. Extremophiles have been identified on Earth that can survive in conditions that were previously considered to be inhospitable to life. Heat, extremely acidic conditions, extreme pressure, and extreme cold are examples of extreme environments. The thermophiles were the first extremophiles to be discovered in the 1960s by Thomas Brock of Indiana University. He was investigating life in Yellowstone National Park’s super-hot water pools. He discovered tiny microorganism mats at Octopus Spring in 1965, when temperatures reached 175 degrees Fahrenheit. Thermus aquaticus was discovered, which led to the discovery of PCR and the creation of a new multibillion-dollar enterprise.

EXTREMOPHILES IN SALINITY: HALOPHILES

The halophiles live in high salt concentrations and are named after the Greek term for “salt-loving.” While the majority of halophiles belong to the archaea domain, some bacterial halophiles and eukaryotic species, such as the alga Dunaliella salina and the fungus Wallemia ichthyophaga, do not. Carotenoid chemicals give certain well-known species, such as bacteriorhodopsin, a red hue. They may be found in salty water bodies such as the Great Salt Lake in Utah, Owens Lake in California, the Dead Sea, and evaporation ponds, where the salt content is more than five times that of the ocean. They’re thought to be a viable contender for extremophiles living in Jupiter’s Europa and other comparable moons’ salty subsurface water oceans.

CELLULAR ADAPTATIONS BY HALOPHILES

High salt-in strategy

The high-salt-in approach protects halophiles from a saline environment by accumulating inorganic ions intracellularly and balancing the salt content in their surroundings through KCl influx. Cl- pumps, which are only found in halophiles and transfer them from the environment into the cytoplasm, are involved in this process. Extreme halophiles of the archaeal and bacterial families keep their osmotic equilibrium by concentrating K + inside their cells. The membrane-bound proton-pump bacteriorhodopsin works to accomplish this.

Low-salt, organic solute-in strategy

The high-salt-in approach necessitates physical modification of all macromolecules in order to survive in a very saline environment, which is incompatible with the survival of moderate halophiles that flourish in salinity-varying environments. Osmolytes protect microbial proteins against dissociation in low-salt water while also improving the bacteria’ tolerance to drastic changes in external saline conditions. Glycine betaine was the first bacterial osmolyte discovered in Halorhodospria halochloris.

The majority of halophiles are unable to thrive outside of their high-salt natural habitats. Many halophiles are so delicate that putting them in distilled water causes them to lyse due to the shift in osmotic circumstances. Halophiles include phototrophic, fermentative, sulfate-reducing, homoacetogenic, and methanogenic species in anaerobic conditions whereas in aerobic conditions include phototrophic, fermentative, sulfate-reducing, homoacetogenic, and methanogenic species.

The Haloarchaea, notably the Halobacteriaceae family, belong to the Archaea domain and make up the bulk of the population in hypersaline settings. The family currently has 15 recognised genera. Bacteria (mostly Salinibacter ruber) can make up to 25% of the prokaryotic community, although it usually makes up a considerably smaller portion of the overall population. In this habitat, the alga Dunaliella salina can sometimes thrive.

EXTREMOPHILES AT LOW NUTRIENT LEVELS: OLIGOTROPHS

An oligotroph is an organism that can survive in a low-nutrient environment. Oligotrophs are usually known for their sluggish development, low metabolic rates, and sparse population density. The settings are ones that provide little in the way of life support. Deep marine sediments, caverns, glacial and polar ice, deep underground soil, aquifers, and leached soils are examples of these habitats.

The cave-dwelling olm the bacteria Pelagibacter ubique, which is the most numerous creature in the seas and lichens with their incredibly low metabolism are all examples of oligotrophic species.

Caulobacter crescentus is an oligotrophic Gram-negative bacteria found in freshwater waterbodies. The whole cell functions as an integrated system in the control circuitry that controls and paces Caulobacter cell cycle development. As it orchestrates activation of cell cycle subsystems and Caulobacter crescentus asymmetric cell division, the control circuitry monitors the environment and the internal status of the cell, including the cell topology. The control system has been meticulously tuned as a whole system for reliable functioning in the face of internal stochastic noise and external unpredictability by evolutionary selection.

The bacterial cell’s control system is organised in a hierarchical manner. The signalling and control subsystem communicates with the outside world through sensory units that are mostly found on the cell surface. To adjust the cell to current conditions, the genetic network logic responds to signals received from the environment as well as internal cell status sensors.

ENVIRONMENT AND LOCATIONS

Oligotrophic lakes are often found in northern Minnesota, with deep clear water, stony or sandy bottoms, and minimal algae.

Oxygen levels are high throughout the water column in oligotrophic lakes. Cold water may store more dissolved oxygen than warm water, thus oligotrophic lakes’ deep regions remain quite cold. Low algal content also provides for more light penetration and less breakdown. Algae, zooplankton, and fish die and are degraded by bacteria and invertebrates at the bottom of the ocean. The process of breakdown consumes oxygen. 

Locations

 Oligotrophs and eutrophs coexist in natural ecosystems, and their proportions are determined by an individual’s capacity to prevail in a given environment.  Despite their capacity to exist in low-nutrient settings, they may struggle to survive in nutritionally- rich ones. Most microorganisms are not well adapted to exist in nutrient-limited circumstances and frigid temperatures (below 5 °C), Antarctic habitats offer very little to sustain life. Some of the documented examples of oligotrophic environments in Antarctica are:

Lake Vostok, a freshwater lake cut off from the rest of the world by 4 kilometres (2.5 miles) of Antarctic ice, is often cited as a prime example of an oligotrophic ecosystem. Because of the lake’s severe oligotrophy, some people assume that sections of it are entirely sterile. This may be used as a model to simulate alien life investigations on frozen planets and other celestial worlds.

Oligotrophic soil environments

In general, nutrient availability decreases as the depth of the soil environment increases, since organic molecules degraded from detritus are swiftly eaten by other microorganisms on the surface, resulting in nutritional deficiency in the deeper levels of soil.

Collimonas is one of those species that may survive in an oligotrophic environment as it has the capacity to not only hydrolyze the chitin generated by fungus for nutrition, but also to create materials. Fungi are a prevalent element of the habitats where Collimonas thrives. In oligotrophic settings, reciprocal relationships are prevalent. Weathering also allows Collimonas to access electron sources from rocks and minerals.

The environment of soil in polar locations, such as the Antarctic and Arctic regions, is termed oligotrophic since the soil is frozen and biological activity is minimal. Actinobacteria, Proteobacteria, and Cyanobacteria are the most common bacteria in frozen soil, with a tiny quantity of archaea and fungus. Under a wide range of low temperatures, actinobacteria can keep their metabolic enzymes active and continue their biochemical processes.

The following are the characteristics that a bacterium should have in order to be labelled as an oligotroph:

(a) Having a form with a high surface-to-volume ratio.

(b) Having an innate propensity for using metabolic energy for food absorption during phases of growth stagnation.

(c) Possessing nutrition absorption abilities that are expressed in a constitutive manner.

(d) Presence of a low-specificity, high-affinity transport mechanism that allows for simultaneous absorption of mixed substrate.

 (e) Having systems for conserving nutrition after it has been absorbed.

Extremophiles and their products have revolutionised many aspects of our home and professional life, from household materials to molecular diagnostics. It is not unlikely that new and medically useful discoveries will be found in the realm of extremophile research; the potential of these organisms is so fresh and huge that their applications may be restricted only by imaginations.

Stage of personality development

Personality is the term used for the integrated and dynamic organisation of the physical, mental, moral and social qualities of an individual. Personality development is very important for all. It helps in all sectors.

Stage of personality development

1 Learning and growth :- Development of people depends upon the work of people in which they have interest according to the age the understanding level of children will be increased by the passing of time.

2 Learning and maturity :- At the age many types of changes comes in the children life. Many types of changes are seen in the children and after that they can understand any thing.

Process of development :- process of development depends upon the daily work of children and from that any one can understand and personality development of children.

Important of personality development

From the personality development any one get confidence from their work. Life is more easier if we do something new for students. It helps us in achieving the goal of our lige. At the time of interview the interviewer 1st see the personality of a person and then after the education. For the success personality development is very important.

History of twitter

Twitter is an American social networking and microblogging service. Twitter users interact with small messages called, “tweets”. Currently twitter has more than 330 million monthly active users. It is in the league of big social media platforms like Facebook, Instagram etc. It was created by Jack Dorsey, Noah Glass, Biz Stone and Even Williams in March. In this article I will take you through the journey of creation and rise of twitter.

Initial history

In 2006, Jack was an undergraduate student at New York University. He came up with an idea of an SMS service to communicate with small group. He shared his idea with board members of a podcasting members Odeo. Williams was also part of board members. He later ascribed the idea to Noah. They decided to name this project twttr as domain twitter.com was already in use. Later they bought the domain name and changed the name of service to twitter. Twitter spun off its own company in 2007.

Work on the project started on March 21, 2006 and full version was publicly in july 2006.

The first breakthrough moment for twitter came in 2007 through South by Southwest Interactive conference. During the event, twitter engagement increased from 20000 tweets par day to 60000. They placed two 60 inch plasma screens in the conference hallways. These screens were used to stream twitter messages.

This conference helped twitter in rapid initial growth. Almost 400k tweets were being posted on twitter per quarter in 2007. This increased to 100 million tweet per quarter in 2008. By 2010, company recorded over 70000 registered applications. In march 2021 it became third highest ranking social site. Prominent events like football world cup, NBA finals etc. helped twitter in growing quickly. When Michael Jackson died on 25th June 2009, twitter severs crashed as users were tweeting at the rate 100k tweets per hour.

Emergence of “New Twitter”

After gaining popularity, twitter started revamping it’s service. Initial changes included the ability to see pictures and videos without leaving twitter by clicking on link given in individual tweets of various sites like YouTube.

On 5 April 2011 , twitter released a new home page but due to some glitch it was relaunched on 20th April 2011. On 8th December 2011, twitter added a new feature to its website named “FLY”. In addition to home tab, the connect and discover were also introduced. On 21 March 2012, twitter celebrated its birthday and also announced that they have 140 million active users.

In 2012 twitter expanded at a fast rate. They opened an office in Detroit to work with automobile brands. In june 2012, logo of twitter was modified to bird symbol. Throughout next couple of years twitter acquired several companies like Vine, Crashlytics, Trendrr etc.

In 2014, twitter underwent a redesign which significantly changes its interface. Some of major changes included profile picture and biography in a column left to the timeline, and a full-width header image. Twitter acquired many different type of companies onwards 2014 like Namo Media ( a native advertising company) in 2014, Mitro( a password-security startup) on 31st july 2014, Niche(an advertising network) on February 2015 etc.

Twitter took an significant step in 2020 and started marking tweets which contain misleading information.

Twitter kept expanding its business and was announced to be the 10th most downloaded mobile app in 2019.

PERMACULTURE-ETHICS,PRINCIPLES,METHODS

BY DAKSHITA NAITHANI

INTRODUCTION TO PERMACULTURE:

Permaculture is a method of design in agriculture that emphasises whole-systems thinking and the use of or stimulation of natural patterns.

Bill Mollison, a senior lecturer in Environmental Psychology at the University of Tasmania, and David Holmgren, a graduate student in the Department of Environmental Design at the Tasmanian College of Advanced Education, coined the term.

These principles are being applied in a growing variety of industries.

HISTORY:

Permaculture as we know it now was created in the 1970s t happened approximately a decade after the world became aware of the risks of pesticides like DDT and the damage they represented to humanity and the environment.

Because it was created for the development of long – term (in other words, permanent) systems, the phrase was coined from a combination of the words “permanent” and “agricultural.”

It was one of the first agricultural systems to recognise that local actions might have drastic implications.

 Holmgren is credited for popularising permaculture but it’s worth mentioning that various books on topics like agroforestry and forest farming have been around since the 1930s or earlier.

3 ETHICS:

Permaculture has 3 core tenants:

•             Care for the earth. To put it another way, assist all living systems in continuing to exist and multiply. But a healthy world is required for existence, it is important to understand the principles of nature and how it functions.

•             Care for the people. Allow people to have access to the resources they require to live. Members of the community who are in need of assistance are supported by the community (e.g. after someone dies, help build homes).

•             Fair share. We should take only what we require and reinvest any excess. Any surplus can be used to assist satisfy the other two basic tenets. This involves reintroducing waste products into the system so that they can be reused.

PRINCIPLES:

All sustainable community design initiatives should use Permaculture concepts.

They are the most important rules for putting it into practise. They may aid in improving and protecting the land, ecosystem, and people, as well as maximising efficiency and productivity.

These principles promote innovation while maximising outcomes. Every location, every circumstance, and every family is unique. As a result, each project’s plans, procedures, plants, animals, and building materials may differ. Even yet, the same principles apply to any location and endeavour, big or little.

1. Observe and Interact

2. Catch and Store Energy

3. Obtain a Yield

4. Apply Self-regulation and Accept Feedback

5. Use and Value Renewable Resources and Services

6. Produce No Waste

7. Design From Patterns to Details

8. Integrate Rather Than Segregate

9. Use Small and Slow Solutions

10. Use and Value Diversity

11. Use Edges and Value The Marginal

12. Creatively Use and Respond to Change

BENEFITS OF PERMACULTURE:

Reduced water usage

Wastewater and rainfall are used in permaculture. This is useful for homes, but for farms with larger areas, it becomes a more cost effective and efficient means of watering the produce.

Reduced waste

Nothing is thrown away. Garden waste, leaves, table scraps, and other waste products are composted or fed to animals as food. Some people go beyond and utilise compost toilets to fully live a zero-waste lifestyle. Permaculture is only sustainable if it makes use of leftovers.

Economically feasible

It is cost effective since pesticides are not required, and most systems require minimal upkeep. All you have to do is water the plants and mulch them once in a while.

Less pollution

Permaculture is a more natural manner of growing food, tractors and other powered agricultural equipment are rarely used.

Improved values

You’ll automatically acquire more ethical and good principles like consuming little, just using what you need, minimising pollution, and helping others if you practise.

More self-sufficiency

A farmer or gardener who practises permaculture may grow a broader range of crops on their property. It allows you to be self-sufficient by allowing you to grow whatever you desire or need to eat.

Applicable to existing systems

 Agricultural systems and lands that already exist can be converted to principles. Permaculture may be practised on a big or small scale wherever that you can normally grow food.

COMMON METHODS OF PERMACULTURE:

1) Agroforestry

Agroforestry is a technique that incorporates trees, shrubs, animals, and crops. The term is derived from a blend of agriculture and forestry. These two apparently disparate professions collaborate to produce systems that are more resilient, healthy, lucrative, and productive. Forestry farming, which is a permaculture technique also falls under the category of agroforestry. However, the main concept is to construct your food forest using a seven-layered method. A canopy layer, a low tree layer, a shrub layer, a herbaceous layer, a rhizosphere, a ground cover layer, and a vertical layer are all included. Silvopastoral and silvoarable are two other agroforestry systems.

2) Hügelkultur

Hügelkultur is a German word that means “hill culture.” It’s a method of burying huge volumes of wood in order to increase the soil’s ability to retain water. This rotting wood behaves like an absorbent, soaking up water from the ground.  Plant materials which behave as a compost are usually placed on top of the mound and decomposed into the soil. A Hügelkultur mound generally lasts 5 to 6 years until the wood rots completely and the procedure must be repeated.

3) Harvesting Rainwater and Grey water

Instead of letting rainwater wash from the property, you may collect it and store it for later use. Roofs gather the majority of rainwater. Eaves troughs, which collect and transport water away from buildings, are likely already installed on your farm’s homes, barns, and other structures. To collect rainwater, just connect a big tank to your downspout and catch the water rather than having it seep into the ground and go to waste. Storm water harvesting is another way to collect water. It is distinct from rainwater harvesting in that it collects runoff from creeks, drains, and other waterways rather than from rooftops. Grey water is a last source of reusable water on the farm. This is water that is used in the house or on the farm for things like bathing and doing laundry.  Because grey water includes detergents, it cannot be used for drinking, but it may be utilised for irrigation purposes and other reasons.

4) Cell Grazing

Grazing is commonly seen as a negative activity that, if not carried out appropriately, has the potential to harm the ecosystem in various ways. Allowing animals to overgraze a region can have severe repercussions, and this is true. Cell grazing is the favoured approach in permaculture. This entails moving herds of animals between fields, pastures, or woodlands on a regular basis. The disruptions created by grazing animals, when done correctly, can actually improve the ecosystem and allow plants to recover more quickly. It also keeps an eye on how animals interact with the land. Plants require appropriate time to rest between each grazing and therefore it’s critical that a region receives a rest time after being grazed.

5) Sheet Mulching

Mulching is simply any protective layer placed on top of the soil to retain water and prevent weed development and is used by many farmers and gardeners. A variety of materials such as wood chips, cardboard, plastic, stones, and are frequently employed. Sheet mulching is an organic no-dig technique that aims to imitate natural soil building in forests, namely how leaves cover the ground. Sheet mulching is most often done with alternating layers of “green” and “brown” materials. Fallen leaves, shredded paper and cardboard, pine needles, wood chips, and straw are examples of brown materials. Manure, grass clippings, worm casings, vegetable scraps, hay, coffee grounds, and compost are examples of green materials. It’s possible to utilise 5 to 10 layers of materials. Sheet mulching adds nutrients and minerals to the soil, inhibits weed development, regulates weather and protects against frost, reduces erosion and evaporation, and absorbs rainwater.

6) Natural Building

Natural building is a more environmentally friendly alternative to purchasing materials from your local hardware shop or lumber yard. You should try to employ as much recycled materials as possible in a system. There are a lot of renewable resources on the land that you may employ in your next construction project. Most people ignore clay, pebbles, wood, reeds, straw, and sand, which are all easily available materials. Tires, which are less natural, can also be utilised for building. This is a fantastic method to recycle old tyres that would otherwise be thrown away or burned. Similarly, instead of purchasing new windows, discarded glass windows are frequently repurposed.

7) No-Till or Minimum-Till Farming

The goal of no-till farming is to leave the soil untouched. The soil is left undisturbed rather than being broken up before planting. This helps to keep water in the soil, keeps carbon from leaving the soil, increases soil quality, and lowers the quantity of weed seeds that are brought closer to the surface to germinate. The soil is disturbed by conventional agriculture methods. This allows carbon dioxide to enter the atmosphere while also over oxygenating the soil. Loosening the soil in this way can cause erosion and nutrient runoff, as well as obliterate important fungal networks. Tilling can be reduced or even removed altogether for some systems with the right approaches.

8) Intercropping and Companion Planting

Intercropping is the planting of more than one two plant species in the same region that mutually benefit one another. Companion planting, for example, involves growing strong-scented plants and herbs such as basil, oregano alongside primary. Many of these companion plants with powerful smells are repulsive to pests. Not only that, but some of them really help the plants they’re partnered with to grow and taste better. Others help to loosen the soil or provide additional advantages. While many plants get along well when grown together, there are some who don’t because they demand the same nutrients or for other reasons.

9) Market Gardening

Market gardening is an intriguing shift away from conventional style of agriculture, which is carried out on huge swaths of land far out in the nation, to smaller plots of land, even in metropolitan areas sometimes. Market gardeners, as the name implies, sell their vegetables at farmer’s markets, however some may also supply restaurants and grocery shops directly.

Cash crops are aggressively produced on a small scale in market gardening (usually less than an acre of land.) While cultivating on as little as a quarter acre of land, a market gardener may earn up to $100,000 each year.

Heath Benefits Of Cycling 15 Minutes A Day

When done properly, cycling is an effective and enjoyable form of aerobic exercise. Cycling can reduce the risk of heart disease, high blood pressure, obesity and diabetes according to a report by Kelley GA. Effects of Aerobic exercise in normotensive adults, 1995. It can reduce your ‘real age’, lowering it more than a decade lower than your chronological age.

The indirect health benefits include reducing serious injuries caused by falls in older people, osteoporosis, and hip fractures.

Statistics show that cyclists, even those who only travel short distances can reduce the risk of death by 22 per cent.

Optimum results are achieved when cyclists are breathing heavily, but are not out of breath. Exercise has been shown to increase HDL (“good” cholesterol) and reduce the amount of triglycerides in the blood. Again, this means improved cardiovascular health. This leads to a reduced chance of heart blockage and reduces the risk of stroke. There are some reports that link exercise to a lowered risk of developing some cancer, like colon cancer.

Cycling burns the calories in a chocolate bar or a couple of alcoholic drinks in one hour, 300 calories. Translated into modern lifestyle terms, a fifteen-minute bike ride, five times a week, burns off 11 pounds of fat a year and meets the requirements for reducing heath risks.

There is a trick to exercising. Running a nine-minute-mile burns 11 calories a minute. Walking at 18 minutes per mile burns five. Faster exercise burns more calories.

Exercise continues to burn fat after the workout ends. Once the sweating stops the body’s metabolisms remains high. You can you increase the post-exercise burn?

A few scientific studies suggest that exercising for 20 minutes at 35 to 55% of aerobic capacity, as in riding briskly, elevated metabolism for 20 minutes after stopping. That means that a 20 minute, brisk bike ride burns fat for 40 minutes.

Cycling has been proven to reduce stress and depression and relieve symptoms of premenstrual syndrome.

The Department of Transport reports that ‘even a small amount of cycling can lead to significant gains in fitness’. The study reveals that aerobic fitness was boosted by 11 per cent after six weeks of cycling ‘short distances’ four times a week and cycling four miles a day the aerobic benefit increased to 17 per cent.

The Fentem PH. ABC of sports medicine report, Benefits of exercise in health and disease, 1994 concludes that cycling is ‘one of the few physical activities which can be undertaken by the majority of the population as part of a daily routine’.

The choice of bike is a personal thing.

Most people never cycle more than five miles, so choosing an expensive bike designed to challenge the most adventurous dare devils. Instead, choose a bike that will manage the local terrain, comfortably.

Equipment needs will also vary. Older people should consider elbow and knee guards. These will help prevent debilitating joint injuries that can seriously limit your mobility. A helmet is not optional.

Do not buy a helmet from a local hardware store. Get one from a bicycle specialty store. The selection is larger, and the quality better. Shopping at an online store can also save money.

Once introduced into the bicyle riding community, you’ll soon set out on your daily cycle for the joy of it. Health benefits will be secondary

ASPERGILLOSIS

BY DAKSHITA NAITHANI

INTRODUCTION

Pier Antonio Micheli, a priest and scientist, named Aspergillus while documenting moulds in 1729. The mould looked like an aspergillum, which is a type of holy water sprinkler. Physician Georg W. Fresenius described the species fumigatus for the first time in 1863.

 Apergillus spp., found in abundance of nature, can be found in soil, decomposing materials, water sources, and air particle. Aspergillus-related disease is uncommon, although it has a significant death rate. The majority of people are exposed to spores (conidia) during their lives, although illness development occurs mostly in people with compromised immune systems, as well as during construction and yard activities. In patients with leukaemia, asthma, cystic fibrosis, and hematopoietic stem cell transplant recipients, A.fumigatus is the most frequent invasive mould infection, with death rates exceeding 50 percent in these high-risk populations.

Around the apex, the stipes are grey in colour. They feature a slick finish. Their globule is tiny and columnar. The conidia’s texture is either smooth or spinose.

WHERE IT CAN BE FOUND?

Aspergillus is commonly found in rotting plant matter. After considerable environmental exposure to Aspergillus spores, such as when handling tree-bark chipping, Aspergillosis can develop in healthy hosts.

Aspergillus can be found in soil, compost piles, and damp grain in outdoor settings. Aspergillus may be found within buildings in wet insulation, fireproofing material, beds , behind couches, in damp rooms’ corners, dust, and air conditioning systems. In the Northern Hemisphere, most research studying seasonal changes in fungal exposure show an increase in airborne Aspergillus throughout the cold season.

TYPES OF ASPERGILLOSIS

Allergic bronchopulmonary aspergillosis (ABPA) is a multifaceted hypersensitivity reaction to inhaled Aspergillus fumigatus. Although it produces lung irritation and allergy symptoms, it does not cause infection. Aspergillus sinusitis is caused by an allergic reaction to the fungus Aspergillus, which produces inflammation in the sinuses and the symptoms of a sinus infection but does not cause an infection. A fungus ball is also known as an Aspergilloma. It forms in the lungs or sinuses but seldom spreads to other regions of the body, as the name implies.

Aspergillosis of the lungs (chronic pulmonary aspergillosis) Aspergilloma, chronic cavitary pulmonary aspergillosis (CCPA), chronic fibrosing pulmonary aspergillosis (CFPA), and Aspergillus nodules are all illnesses classified as chronic pulmonary aspergillosis (CPA). CPA is a lung infection that develops over time.

INVASIVE PULMONARY ASPERGILLOSIS

Inhaled conidia are removed by epithelial cells and alveolar macrophages in normal host lungs. Aspergillus becomes invasive when conidia escape these host defences and develop into branching filaments called hyphae. Alveolar macrophages generate inflammatory mediators, which attract neutrophils, which can destroy the hyphae.

CUTANEOUS SKIN ASPERGILLOS

Aspergillus comes in contact through a breach in the skin (for example, after surgery or a burn wound) and infects persons with weaker immune systems. Cutaneous Aspergillosis can also develop if invasive Aspergillosis moves from another part of the body, such as the lungs, to the skin.

LIFE CYCLE

Aspergillus begins its infectious life cycle by producing conidia (asexual spores) that are easily transported into the atmosphere, ensuring its ubiquity in both habitats. Inhalation of these conidia is the most common way to become infected, followed by conidial deposition in the bronchiole spaces. The phagocytosis and destruction of Aspergillus conidia is largely carried out by macrophages. The danger of infection stems largely from a breakdown in these host defences, as well as fungal characteristics that allow A. fumigatus to survive and flourish in this pulmonary habitat.

SYMPTOMS

The symptoms of Aspergillosis are:

  • Wheezing, chest pain
  • Shortness of breath, stuffiness, runny nose and reduced ability to smell
  • Cough (sometimes blood)
  • Fever (in rare cases), headache, fatigue
  •        Weight loss

•             The length of time that an illness lasts depends on the underlying health concerns, the intensity or location of infection, and the kind of sickness.

•             Allergic forms, may require steroid and antifungal treatment for a few months, possibly longer sometimes.       

•             Invasive pulmonary Aspergillosis may need at least 6 to 12 weeks of antifungal therapy. Severe cases may need therapy for lifetime and monitoring to keep the illness under control. 

•             If the infection spreads from the lungs to other areas of the body, additional symptoms may appear.

•             Red to purple plaques or papules are common symptoms of cutaneous Aspergillosis.

DIAGONOSIS    

Because the symptoms of Aspergillosis might be confused with those of other lung diseases, diagnosing it can be challenging. In order to make a diagnosis, your healthcare practitioner will look at your medical history, risk factors, symptoms, physical examinations, and lab testing.

An Aspergilloma, a fungal tumour, or the symptoms of invasive Aspergillosis and ABPA can be found on a chest X-Ray or a chest CT scan.

•             A sample of your sputum is stained with a dye and examined for the presence of Aspergillus filaments in a respiratory secretion (sputum) test.

•             Tissue and blood testing may be used to confirm the presence of ABPA.

•             To confirm invasive aspergillosis, a biopsy of tissues from your lungs or sinuses is performed, as well as blood testing.

TREATMENT

In addition to early diagnosis, early treatment is critical in managing aspergillosis. Depending on the type of disease, one may have the following treatments:

•             Observation- If you have a single aspergilloma, you may not need treatment; instead, you may be monitored with regular chest X-rays or CT scans. The doctor may prescribe antifungal medication if your disease worsens.

•             Antifungal medicines may be used in combination with corticosteroids to reduce the need of steroids, improve lung function, and prevent existing asthma or cystic fibrosis from deteriorating. Some examples are prednisone (Deltasone), prednisolone (Orapred), and methylprednisolone (Medrol).

•               Invasive pulmonary aspergillosis is treated with antifungal medications. Voriconazole (VFEND) and amphotericin B are two of these drugs (Amphocin, Fungizone).

•             Surgery- as antifungal medicines have a hard time penetrating an aspergilloma, surgery may be required to remove the fungal mass if the aspergilloma causes pulmonary haemorrhage.

Embolization of the Arteries -This technique can be used to temporarily halt aspergilloma-related bleeding.  A radiologist uses a catheter to inject a substance into an artery supplying a lung cavity where an aspergilloma is causing blood loss. The injected substance solidifies, cutting off the area’s blood supply and halting the bleeding. This therapy works for a while, but the bleeding will most likely return.